P. N. Mane ¹, N. S. Satpute ², M. P. Moharil ³, S. M. Thakare ², Swapnil Gaikwad ¹, A. K. Gade ¹, M. K. Rai ⁴

Affiliations
1 Oilseeds Research Unit, Dr. Panjabrao Deshmukh Krishi Vidyapeeth, Krishinagar, Akola 444 104. Maharashtra, IN
2 Department of Entomology, Dr. Panjabrao Deshmukh Krishi Vidyapeeth, Krishinagar, Akola 444 104. Maharashtra, IN
3 Biotechnology Centre, Dr. Panjabrao Deshmukh Krishi Vidyapeeth, Krishinagar, Akola 444 104. Maharashtra, IN
4 Department of Biotechnology, Sant Gadgebaba University, Amravati, Maharashtra, IN

Abstract

Present investigation was carried out to test the potency of Silver nanoparticles (SNPs) as UV protectants along with tinopal and mango leaf extract at Department of Entomology, Dr. Panjabrao Deshmukh Krishi Vidyapeeth, Akola during 2011 - 2013. Formulated viral suspension was exposed to UV 20 W Philips lamp (Type 05, range 285 - 320 nm) for 15 minutes and to sunlight at noon hours for 30 minutes,1, 1.5 and 2 hours and determined the viability of POBs and larvicidal activity of viral formulations before and after UV lamp and sunlight exposure. Least reduction in POBs i.e 5.2 per cent was observed from HaNPV + Tinopal 1 %, also recording 86.67 per cent larval mortality when exposed to UV lamp, which was at par with HaNPV alone unirradiated. HaNPV alone when irradiated recorded 55.1 per cent reduction in POBs. HaNPV + tinopal 1 % when exposed to sunlight, protected the POBs upto 1.5 hour, recorded 20.5 per cent reduction in POBs and 83.89 per cent larval mortality. When exposed to 2 hours sunlight reduced the insecticidal activity of HaNPV even though tinopal 1% was used as UV protectant with HaNPV. Of the additives, tinopal 1 % was found to be most effective as it protected the POBs from UV degradation and enhanced larval mortality as compared to mango leaf extract 1 % and SNPs.

Keywords

Silver Nanoparticles, Hanpv, UV Protectants, Tinopal 1 %, Mango Leaf Extract.

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P. N. Mane ¹, M. P. Moharil ², N. S. Satpute ³, S. M. Thakare ³, G. K. Giri ⁴, Swapnil Gaikwad ⁵, A. K. Gade ⁵, M. K. Rai ⁵

Affiliations
1 Oilseeds Research Unit, Dr. Panjabrao Deshmukh Krishi Vidyapeeth, Krishinagar, Akola - 444 104, Maharashtra, IN
2 Biotechnology Centre, Dr. Panjabrao Deshmukh Krishi Vidyapeeth, Krishinagar, Akola - 444 104, Maharashtra, IN
3 Department of Entomology, Dr. Panjabrao Deshmukh Krishi Vidyapeeth, Krishinagar, Akola - 444 104, Maharashtra, IN
4 Department of Plant Pathology, Dr. Panjabrao Deshmukh Krishi Vidyapeeth, Krishinagar, Akola - 444 104, Maharashtra, IN
5 Department of Biotechnology, Sant Gadgebaba University, Amravati, Maharashtra, IN

Abstract

Experiment on storage stability and performance of aqueous and dry formulation of Helicoverpa armigera nuclear polyhedrosis virus (HaNPV) was conducted during 2011-2013 in Bio control laboratory, Department of Entomology and Insect Biotechnology laboratory, Biotechnology Centre, Dr. Panjabrao Deshmukh Krishi Vidyapeeth, Akola with an objective to develop potent HaNPV formulation for improving shelf life and performance. Aqueous and dry form of HaNPV formulations with antimicrobials, UV protectant and phagostimulant were prepared and studied their shelf life and performance during storage. Synthesized and characterized the Silver nanoparticles. Prepared fresh Ha NPV required for preparing different formulation. The contaminants associated, storage stability and larvicidal activity of formulated HaNPV was studied. Data revealed that, HaNPV formulations having Silver nanoparticles @ 8 μl/ml of HaNPV and 80 μl/ml of HaNPV checked the bacterial contamination up to 13 months of the storage period, did not affect the viability of POBs and insecticidal properties of HaNPV formulation. Aqueous form of HaNPV + Streptomycin @ 0.18 g/lit of HaNPV + Tinopal 1% + Sucrose 1% , dry form of HaNPV + Streptomycin @ 0.18 g/lit of HaNPV + Tinopal 1% + Sucrose 1% and HaNPV + Streptomycin @ 0.18 g/lit of HaNPV + sucrose remain stabled up to 12 months. However, aqueous form of HaNPV alone reduces the insecticidal properties from the 9th month of storage period. While, dry form of HaNPV alone reduces the insecticidal properties from the 12th month of storage period. From the result, it was noticed that dry form of HaNPV formulation having antimicrobial found more stable than aqueous form.

Keywords

Shelf Life, Storage Stability, HaNPV Formulation, Silver Nano Particles.

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Amol Waghmare ¹, Niraj S. Satpute ¹, S. K. Bhalkare ¹, P. N. Mane ¹

Affiliations
1 Biocontrol Laboratory, Department of Entomology, Dr. Panjabrao Deshmukh Krishi Vidyapeeth, Akola-444 104 (MS), Maharashtra, IN

Abstract

Influence of rearing temperature on biological parameters of Trichogramma chilonis (Ishii) was studied under laboratory conditions during 2017-18. Maximum per cent parasitization and adult emergence of 74.2% and 78.91%, respectively was observed at 25+1°C followed by 71.8% and 65.21 % at 30±1°C. However, the temperature of 40+1°C was found detrimental to T. chilonis recording only 20.2% parasitization, the progeny of which has failed to complete the development and the adults could not emerge out. The developmental period was maximum (8 days) at 25+1°C. Also, maximum male and female longevity (5 and 12 Days, respectively) and maximum mean per day fecundity of 17 eggs per female/day were recorded at 25+1°C. The progeny was found to be male dominated with a female-male ratio of T. chilonis to be 0.69, 0.74 and 0.96 at 25, 30 and 35°C, respectively.

Keywords

Biological Parameters, BOD Incubator, Rearing Temperatures, Trichogramma chilonis

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P. N. MANE ¹, M. P. MOHARIL ², N. S. SATPUTE ³, D. B. UNDIRWADE ³

Affiliations
1 Oilseeds Research Unit, Dr. Panjabrao Deshmukh Krishi Vidyapeeth, Krishinagar, Akola – 444 104, Maharashtra, IN
2 Biotechnology Centre, Dr. Panjabrao Deshmukh Krishi Vidyapeeth, Krishinagar, Akola – 444 104, Maharashtra, IN
3 Department of Entomology, Dr. Panjabrao Deshmukh Krishi Vidyapeeth, Krishinagar, Akola – 444 104, Maharashtra, IN

Abstract

Experiment was conducted at Department of Entomology, Dr. Panjabrao Deshmukh Krishi Vidyapeeth, Akola during 2011- 2013 to see the effect of natural sunlight (UV) on HearNPV formulation. Experiment was laid out in Completely Randomized Block Design replicated thrice. The aqueous and dry form of HearNPV formulations were prepared by using additives viz. Tinopal an optical brightener and silver nano particle and evaluated their capability to protect NPVs from Ultra Violet rays. HearNPV formulation were sprayed uniformly on the potted chickpea plants during noon hours. Twigs were collected at thirty minutes, One hour, One and half hours and two hours after spraying and kept in petriplates. The laboratory reared second instar larvae of H. armigera were released on each treated twig. Larval mortality was recorded at 4, 7 and 10 days after treatment. An aqueous form of HearNPV formulation, HearNPV + Silver Nano Particles @ 8 µl /ml of HearNPV + Tinopal 1% + Sucrose 1% (T1) recorded 83.04% larval mortality at ten days after spraying which was at par with HearNPV alone unirradiated (84.21 % larval mortality) when exposed to sunlight up to one hour. Among the lyophilized form of formulations, HearNPV + Silver Nano Particles @ 8µl/ml of HearNPV + Tinopal 1% + Sucrose1% (T6) when exposed to sunlight up to one hour recorded 84.80% larval mortality at ten days after spraying which was at par with which was at par with HearNPV alone unirradiated (T12) (85.38% larval mortality) and higher than HearNPV alone irradiated. Both aqueous and lyophilized form of HearNPV showed decreasing larval mortality as compared to HearNPV alone unirradiated when exposed to sunlight up to one and half and two hours. Lyophilized HearNPV formulations recorded higher larval mortality as compared to aqueous form of HearNPV formulations at all sunlight exposure period.

Keywords

HearNPV formulations, Helicoverpa armigera, UV Rays.

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